Name / Stock Number: WiscDsLox288E11 / CS850265

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N850265

seed

available

  • Rick Amasino
  • Michael Sussman
  • Patrick Krysan
  • 09/21/2004

    12/01/2004

    Description:

    Sequence-indexed T-DNA insertion line; the T-DNA includes a Ds transposon and a LoxP recombination site. A Basta resistance marker is used to select for the T-DNA and for the Ds transposon following any transposition. However, be aware that lines from this set might not be able to launch their Ds element. Be sure to test any line for the ability to transpose before using it in experiments. Hygromycin resistance can be used for selection of lines where the Ds element has been excised by transposition. WiscDsLox lines do not contain Ac transposase, and the lines should be crossed to a line expressing Ac transposase in order to mobilize the Ds transposon. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

    none

    hygromycin

    Col (Columbia)

    100 seeds per vial

    $13 / $104

    Germplasm

    2

    Arabidopsis thaliana 3702

    Additional Information

    Transgene Description Promoter Reporter Marker
    pDs-Lox T-DNA includes a Ds transposon and a LoxP recombination site CaMV35S, MAS LUC hygromycin; BASTA

    Quality Control Comments

    Add your quality control comment here

    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here