donor stock number: Ds5 392-13
NASC stock number: N8525
Resource Type: seed
Availability: available
Donors:- Nina Fedoroff
Donation Date: 08/26/1996
Date Released: 08/26/1996
Description:
transposon T-DNA line: carries a single copy transposon T-DNA (Ds-GUS T-DNA); the transposon is derived from McClintock's maize Activator (Ac) transposable element; utilizes a beta-glucuronidase (GUS) gene in a promoter/enhancer trap construct; bacterial aph4 gene (hygromycin resistance) is included as a selectable marker; the transposon is inserted in an herbicide-resistant acetolactate synthase gene, whose function is restored when the transposon excises; map location - chrom. 5, between mi70 and g2368; donated seeds were the R3 generation from a single, homozygous, self-pollinated R2 plant; please consult the Plant Transposon Lab web site for a map of the Ds-GUS T-DNA insertions, information on the use of these stocks and results from transposition experiments; to be crossed to Ac stocks CS8533-CS8538 to induce transposition.
Growth Requirement: none
Marker: hygromycin
Background: No (Nossen)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
transposon insertion
Arabidopsis thaliana 3702
Additional Information
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
Ds5 GUS | enhancer trap transposon | GUS | hygromycin |
Quality Control Comments
There is no quality control data for this stock.