donor stock number: Ac-Rlc-63
NASC stock number: N859431
Resource Type: seed
Availability: available
Donors:- Nina Fedoroff
Donation Date: 02/08/2005
Date Released: 03/21/2006
Description:
Ac-Rlc-63 line; the Ac transposase is supplied by an Ac transposon truncated at the NaeI upstream of the transposase ORF and expressed from a 35S promoter; contains a bacterial neomycin phosphotransferase (NPTII) gene expressed from a mannopine synthase (mas) promoter and followed by a mas 3' transcriptional terminator that serves as the plant transformation marker conferring kanamycin-resistance; the T-DNA vector also carries the agrobacterial tms2 gene as a negative selectable marker (that confers sensitivity to naphthalene acetamide NAM) and the Rlc gene as a visual marker (Rlc gene is a maize transcriptional activator in the anthocyanin biosynthetic pathway).
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment: ABRC has not checked this line for transposase activity; it is suggested that this activity be checked before using in crosses.
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Plants containing Ac transposase can be identified by the expression of the Rlc gene, showing three distinct phenotypes detected from the time of true leaf emergence: 1) increased number of trichomes, which gives plants a hirsute appearance, 2) red to purple color due to increased anthocyanin pigment production and 3) plants grow more slowly than wild type plants.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1023/B:PLAN.0000009271.08420.d9 | 14756312 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Ac-Rlc | Ac transposon truncated at the NaeI upstream of the transposase ORF and expressed from a 35S promoter; contains a bacterial neomycin phosphotransferase (NPTII) gene expressed from a mannopine synthase (mas) promoter and followed by a mas 3' transcriptional terminator that serves as the plant transformation marker conferring kanamycin-resistance; the T-DNA vector also carries the agrobacterial tms2 gene as a negative selectable marker (that confers sensitivity to naphthalene acetamide NAM) and the Rlc gene as a visual marker | kanamycin |
Quality Control Comments
There is no quality control data for this stock.