NASC stock number: N879698
Resource Type: seed
Availability: available, note that a confirmed line has been isolated from this stock
Donors:- Syngenta, USA
Donation Date: 04/06/2006
Date Released: 06/21/2006
Description:
T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.004630 | 12468722 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pDAP101 | T-DNA | BASTA |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Researcher reported low germination | ABRC | 12/09/2016 | To view detailed data, click here |
| Previous low germination stock was replaced with a stock that the germination was found to be 90%. | ABRC | 02/28/2018 | To view detailed data, click here |