donor stock number: DW150.12
NASC stock number: N8849
Resource Type: seed
Availability: available
Donors:- Frederick Hempel
Donation Date: 03/03/1998
Date Released: 06/28/1999
Description:
Transgenic line; LFY::GUS, contains pDW150 derived from pDW137, a pCGN1547 derivative containing the HindIII-EcoRI fragment of pBI101.2, which encompasses the B-glucuronidase (GUS) coding region and the nos terminator, the 2.3 kb BamHI fragment spanning the LFY promoter was inserted in front of GUS in pDW137, such that the fusion gene uses the LFY initiation codon, which overlaps the downstream BamHI site; LFY expression detected before floral determination, with gradual changes during the vegetative phase of plant development, changing quantitatively during the photoinduced transition to flowering. Heterozygous for the transgene. Kanamycin resistant.
Growth Requirement: kanamycin resistant
Marker: kanamycin
Background: No (Nossen)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
LFY::GUS expression detected before floral determination, with gradual changes during the vegetative phase of plant development, changing quantitatively during the photoinduced transition to flowering.
segregating
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| LFY::GUS-FH | the 2.3 kb BamHI fragment spanning the LFY promoter was inserted in front of GUS in pDW137, such that the fusion gene uses the LFY initiation codon, which overlaps the downstream BamHI site | LFY | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.