Arabidopsis Biological Resource Center

Description:

mapping population for tetrad analysis; a backcross population of the F1 of qrt1-1, Ler-0 (CS8845) by qrt1-2, Col-3 (CS8846) to ms1-1, Ler-0; homozygous alleles of qrt1 were utilized in both parents to produce F1 plants with pollen tetrads; the ms1 allele of the backcross parent facilitated hybridizations; the backcross parent resulted from crossing between ms1-1, Ler-0 (CS75) and gl1-1, Ler-0 (CS64); single pollen grains were isolated from tetrads and utilized in separate pollinations; progeny plants were obtained and included in the mapping population from at least three members of each tetrad set; this mapping population consists of selfed progeny from 292 backcross families, 97 tetrads and 2 parental lines; there is segregation within and among the lines for qrt1-1 vs. qrt1-2 and er1-1 vs. ER; this population was used by the donors to map the centromeres and analyze gene conversion.

Growth Requirement: none

Marker:

Background: Col (Columbia); Ler (Landsberg erecta)

ABRC Comment: the parental lines are included with this stock.

Format Shipped: 100 seeds per vial

Base / Commercial Price: $880 / $7040