donor stock number: Q0681
NASC stock number: N9210
Resource Type: seed
Availability: available
Donors:- Jim Haseloff
Donation Date: 01/10/2000
Date Released: 01/10/2000
Description:
GFP line; part of teaching set; use for root lab
Growth Requirement: none
Marker:
Background: C24
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
GFP expressed in the lateral root cap cells, patchy in the rest of epidermis, but getting stronger in more mature root, with strong expression near the hypocotyl. Also expressed in leaf margins.
T-DNA insertion
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pBIN m-gfp5-ER | enhancer trap T-DNA derived from pBI121; contains a BAMH1-SST1 fragment carrying a modified GFP gene with ER retention singnal | GFP | kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Researcher reported a problem with germination | user | 02/11/2025 | To view detailed data, click here |
| Germination rate was found to be 11%; this stock will be replanted to increase germination. Until the stock is replanted, an adjusted higher number of seeds will be sent for this stock. | ABRC | 02/25/2025 | To view detailed data, click here |