Arabidopsis Biological Resource Center

Description:

Transgenic line; Plants were transformed with Agrobacterium tumefasciens (GV3101) using the floral dip infiltration method. A modified pGPTV-BAR binary vector, pL700, was used for transformation. The Medicago truncatula hydroperoxide lyase E cDNA (without a putative N terminal regulatory sequence) was cloned into the Gateway compatible pDESTL700 plasmid. The uidA gene was excised from the pGPTV-BAR binary vector with HindIII and SstI and replaced with potato light-inducible tissue-specific ST-LS1 gene promoter (bases 73-1574 of accession X04753). In addition, a polylinker with the BlnII, SmaI, SpeI, XhoI, and BsiWI restriction sites was added between the promoter and the nos terminator. The pL700 vector was made into a Gateway (Invitrogen) destination vector (pDESTL700) by ligating the Gateway cassette A (attR1: chloramphenicol resistance gene: ccdB negative selection gene: attR2) into the SmaI site. Restriction enzyme digest analysis indicated the cassette was cloned in the correct orientation. The transgene containing MtHPL-E results in overexpression of HPL. Determined to be homozygous for a single transgene on the basis of segregation. Basta resistant.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120