NASC stock number: N9786
donor stock number: MtHPL E-3-4
Resource Type: seed
Availability: not available
Donors:- Eric Belfield
- Rod Casey
Donation Date: 12/04/2018
Date Released:
Description:
Transgenic line; Plants were transformed with Agrobacterium tumefasciens (GV3101) using the floral dip infiltration method. A modified pGPTV-BAR binary vector, pL700, was used for transformation. The Medicago truncatula hydroperoxide lyase E cDNA (without a putative N terminal regulatory sequence) was cloned into the Gateway compatible pDESTL700 plasmid. The uidA gene was excised from the pGPTV-BAR binary vector with HindIII and SstI and replaced with potato light-inducible tissue-specific ST-LS1 gene promoter (bases 73-1574 of accession X04753). In addition, a polylinker with the BlnII, SmaI, SpeI, XhoI, and BsiWI restriction sites was added between the promoter and the nos terminator. The pL700 vector was made into a Gateway destination vector (pDESTL700) by ligating the Gateway cassette A (attR1: chloramphenicol resistance gene: ccdB negative selection gene: attR2) into the SmaI site. Restriction enzyme digest analysis indicated the cassette was cloned in the correct orientation. The transgene containing MtHPL-E results in overexpression of HPL. Determined to be homozygous for a single transgene on the basis of segregation.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
altered aldehyde profile
Arabidopsis thaliana 3702
Additional Information
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
MtHPL E | Medicago truncatula hydroperoxide lyase E cDNA (with a putative N terminal regulatory sequence) with potato light-inducible tissue-specific ST-LS1 gene promoter | potato light inducible tissue specific | BASTA |
Quality Control Comments
There is no quality control data for this stock.